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Order NO. D191001
小鼠抗6X His单克隆抗体
英文名 Anti-6×His Tag mouse monoclonal antibody
别名 6 His epitope tag
相关类别 标签抗体 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 Fusion proteins containing the His Tag
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 6×His Tag    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D191001-0025 25 ul 现货
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D191001-0100 100 ul 现货
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D191001-0200 200 ul 现货
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技术规格

Background: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Applications: WB, IF, IP
Name of antibody: 6×His Tag
Immunogen: Synthetic peptide conjugated with KLH
Full name: HHHHHH Tag
Synonyms: 6 His epitope tag
WB Predicted band size: 50 kDa
WB Positive control: Fusion proteins containing the His Tag
WB Recommended dilution: 1000-5000
IF Positive control: 3T3 cells
IF Recommended dilution: 100-500
IP Recommended dilution: 100-200
相关文档
Sample Application

  • Gel: 8%SDS-PAGE, Lysate: 2 μg, Lane: Fusion proteins containing the His Tag, Primary antibody: D191001(6×His Tag Antibody) at dilution 1/3000, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 1 minute

  • The image is immunofluorescence of methanol-fixed 3T3 cells transfected with His-TAF4B vector using D191001(6×His Tag Antibody) at dilution 1/500

  • Immunoprecipitation analysis: Lane 1 is Transfected cell lysate(Input), Lane 2 is Transfected cell lysate using D191001(6×His Tag Antibody) at dilution 1/200, Lane 3 is Normal Mouse IgG(Control)
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