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Order NO. D199973
小鼠抗eRFP单克隆抗体
英文名 Anti-eRFP mouse monoclonal antibody
相关类别 标签抗体 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 eRFP
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 eRFP    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199973-0100 100 ul 预计5日内发货
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技术规格

Background: Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
Applications: WB
Name of antibody: eRFP
Immunogen: Purified mRFP protein expressed in HEK293 cell
WB Predicted band size: 27 kDa
WB Positive control: HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRFP ne) cDNA for 48 hrs and lysed
WB Recommended dilution: 1000-5000
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Sample Application

  • Gel: 12%SDS-PAGE, Lysate: 5μg, Lane 1-2: HEK293T cells lysates were transfected with the pCMV6-ENTRY control and pCMV6-ENTRY eRFP cDNA for 48 hrs, Primary antibody: D199973(eRFP Antibody) at dilution 1/2000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 2 minutes
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