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Order NO. D199987
小鼠抗His单克隆抗体
英文名 Anti-His mouse monoclonal antibody
别名 6 His epitope tag
相关类别 标签抗体 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 His tag
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 His    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199987-0100 100 ul 预计5日内发货 暂不支持线上订购

技术规格

Background: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Applications: WB
Name of antibody: His
Immunogen: synthetic peptide (HHHHHH) coupled to KLH
Synonyms: 6 His epitope tag
WB Positive control: six different HEK293T over-expression lysates containing different His tagged recombinant proteins, and two his tagged bacterial lysate produced in E.coli.
WB Recommended dilution: 2000-10000
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